Home > Sandwich ELISA Kits > Human Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit

Product Name:

Human Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit

Cat.#:

JL41894

Regulatory Status:

RUO

Alternative:

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.04ng/mL

Specificity:

This kit recognizes Human CAMPin samples. No significant cross-reactivity or interference between Human CAMP and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| CAMP

Cathelicidin antimicrobial peptide (CAMP) is an antimicrobial peptide encoded in the human by the CAMP gene.The active form is LL-37. In humans, CAMP encodes the peptide precursor CAP-18 (18 kDa), which is processed by proteinase 3-mediated extracellular cleavage into the active form LL-37.The cathelicidin family includes 30 types of which LL-37 is the only cathelicidin in the human.Cathelicidins are stored in the secretory granules of neutrophils and macrophages and can be released following activation by leukocytes.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.09	1.54	1.12	0.77	0.48	0.34	0.25	0.09
Corrected OD	2	1.45	1.03	0.68	0.39	0.25	0.16	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CAMP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	93-105	102

| Linearity

Samples were spiked with high concentrations of Human CAMP and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	92	93
1 : 4	Range (%)	89-103	86-108
1 : 4	Average (%)	94	98